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Microscopy of moving white blood cells

Microscopy of moving white blood cells


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Is there a way to get white blood cells to move while on a microscope slide? I want to try to get some microscope images of them moving, but so far I have only found them stationary.


Not an experimentalist, so take this with a grain of salt. But for motility you often need some external factor, e.g. a chemokine or chemoattractant (FMLP is a common one for white blood cells). Also some cell types may not crawl on bare glass, and might need a protein or gelatin coating.

White blood cells on slides can look like this: https://www.youtube.com/watch?v=JnlULOjUhSQ

Unfortunately, I don't know the conditions used there. My recommendation is to look through Google Scholar for white blood cell motility or neutrophil motility, and see what turns up. For instance, the first paper I found was: C S Spada, A L Nieves, A H Krauss and D F Woodward, J. Leuk. Biol. 1994 (PDF) which gives some details about how they got their assays to work, including a gelatin coating for the slides. However, the details probably depend on the origin of your cells and the type of white blood cell you're looking at.


White blood cells move. As long as they're alive, they move, but they do it veeeeeery slowly. The movements can be seen using time-lapse photography. See: https://www.wetenschapsforum.nl/index.php/topic/199938-levend-bloed-onder-de-microscoop/

These were made at about 1,000x, anoptral contrast.


Microscopy of moving white blood cells - Biology

Live blood microscopy in a person suffering from Morgellons Disease

On Sept. 8, 2012 I had a live blood microscopy with Rick Panson, a microbiologist, at the Live-Live store in NYC. Rick has been doing live blood analysis since 2005 and works as a healthy body coach. He spent an hour going over what he saw in my blood as well as giving me pointers that would move me into a greater state of health. It was incredibly informative helping me explore new directions to pursue. It is one thing to have lab work and testing, and quite another to witness firsthand what is transpiring in your blood. Many thanks to Rick for going the extra mile and providing photos. His comments are in red and italics.

On Nov. 16, 2012 I saw Rick again for a second session. The results are posted following the chia seed information.

At the bottom of the page are images that various people suffering with Morgellons graciously contributed to this site.

A note on the photos. Rick provided me with photos for this page and I've filled in some of the gaps with screen grabs taken from the video that was taping our session.

Nov. 8, 2012 • 1st live blood microscopy session 

Left:
Heterogeneous symplasts The tiny white dots in the clump are yeast/candida. There are several stages of microorganisms here which include yeast, bacteria and fibrin, fungus and mold. A condition of acidosis is causing this.


Left and below:
Ghost cells
The dark black cells with the halos are red blood cells. In the background are 'shadow cells' which were healthy red blood cells that 'popped' due to the loss of their phospolipid membranes. This can occur from an acid producing diet, and poor assimilation of nutrients.

Rick's comments "Healthy blood cells will be perfectly round, nice and dark with a glowing halo around them. Usually, if they have enough moisture inside them they are fully round because there is more pressure inside the cell then outside."

"These 'shadow' or ghost cells were once perfectly healthy cells, you can see by their shape and color that they were—and they've now disappeared into the background. They've popped, they lost their phospholipid membrane. Every cell in the body has a double membrane of fat made up of phospholipids, a two layer membrane. The acids can disentegrate the cell membrane as well as when your body ferments sugar, alcohol is the byproduct, and alcohol can easily melt the cell membrane."

"Whatever is happening in the blood cells is happening to the rest of the body. This will make one tired."


(Rick asked if I bruise easily and that answer was YES!). His recommendation was the addition of chia seeds to the diet. Chia is high in Omega 3 fatty acids and are 28% protein and 40-60% fatty acids. More on chia below.

Above: Rouleaux Sticky or stacking chaining of the red blood cells, it's related to poor protein, metabolism and altered pH or acid imbalance, which varies the electrical negative charge of the cell membrane causing them to stick together, rendering red blood cells unable to pass into the small capillaries to transfer oxygen or biologically transform into new body cells and/or remove carbon dioxide or other metabolic acids resulting in fatigue & tiredness, poor circulation and weak body cells.

"One combats sticky blood by ionized water which has massive negative electrical charge which gives its energy to the cells and when properly "energized" they all have a negative electric charge and thus repel each other rather than stack and chain."

Above and left: Erythrocyte (RBC) aggregation . Clotted mass of blood cells due to changes in the cells polarity, loss of natural negative electrical charge. Basically it's a blood clot happening in the system caused by dehydration.

From the literature I was given: "RBCs clumping together in a mass due to a change in the negative surface charge this is a more disorganizing symptomology where plasma acts as molecular glue, causing RBCs to stick together. This results in poor circulation that leads to cold hands, cold feet, increased body temperature, sweating, hot flashes, water retention, bloating, lightheadedness, dizziness, muddled thinking, forgetfulness and fatigue."

Above: The spaces in between the cells are revealing the hydration level (or lack of). When there are this many cells and this little water, we are looking at the hydration level of the body. I'm dehydrated.

Above: The little black dots are fibrin spicules (bacterial balls) and the cross-hatching pattern in the background that looks like tiny cracks is 'fibrin nets' or 'trees.' Indicates a high level of latent tissue acidosis.

"When the little bacterial balls become more advanced and accumulated, the fibrin nets, like cobwebs form. First come the bacteria—then the nets. These nets act as a defense mechanism for the bacteria so that the white blood cells can't get to them. This system of netting is very difficult on the cardiovascular system because it acts like a cobweb, slowing things down and creating congestion, as well as raising blood pressure."


Rick's comments on photos below: "You're gonna want to take a picture of that!"
He did not know what it was and has only seen something like this once before
in his career.

"My first guess would be it's a piece of lint caught between the slides that has nothing to do with your blood. A piece of 'non-you'—because we are not working in a real sterile environment here. It doesn't seem to be biological. Let's call it a fiber. for now. It doesn't belong there, that's for sure. There is no doubt that that is not part of normal healthy blood, and it's way bigger then what I think could survive in your cardiovascular system without being filtered out by your filter organs. That's why I don't think it's a fiber, per se, because it's so big compared to everything else. From a biology point of view, my best guess is that it would be a contaminent that has nothing to do with you. We'll see if we see another one, because if we do, it's clearly not a contaminant—because the chances of there being two pieces of dust on that slide after I wiped everything fresh are slim." We did not see another fiber.


Below:
some more shots of the 'artifact' as it winds around in the blood.

Above: All those white globs in the middle of the cells are yeast consuming the red blood cell—Candida. The red blood cells are fermenting as the yeast eats the cell itself.


Above: Neutrophils You go guys! The 'garbage collectors' doing a valiant job of gathering yeast and bacteria. They were very active, a good sign. At least there is some good news.
My immune system is working.


Above: Yeast The arrow on the right is showing yeast being pushed from the red blood cell. The other arrow is free-floating yeast, and you can see there is quite a bit of it in the
blood. This is due to acid imbalance. Top right you can see heterogeneous symplasts forming.


Above: More Rouleax . Sticky, sticky blood, red blood cells stacking


Above: Sugar intolerance Those short lines should be continuing throughout the blood and are not—which indicates sugar intolerance.


Above: Black particles are heavy metals

Left:
Low alkaline buffers
That whole pale ridge should have snapped to the darker edge.

"Low alkaline buffers means that you don't have enough of the alkaline minerals (sodium, magnesium, potasium and calcium). Sodium bicarbonate (baking soda) is the most important and most effective alkalizer
your body makes to try and keep alkaline balance.
When you consume baking soda into digestive system, much of it simply neutralizes your hydrochloric acid in stomach whereas when you take it sub-lingual (under the tongue) it neutralizes acids in the body tissues—mostly carbonic acid from carbon dioxide."


Above: This tiny section of the blood is how all the dried blood would look if it were healthy. There is very little ledge. That's what we are aiming for.


Above: Little white dots in the center are liver stress probably due to the fermentation taking place inside the body because of the alcohol, not that much to worry about there.

Above: White dots in the middle is bowel toxicity. More of that in image below.

I left the consultation with some really good pointers. Rick recommended purchasing a GoldFox water ionizer. This is not the greatest water filter but what it does is alkaline the water, so instead of drinking water that is pH 5 or 6, one is drinking water with a pH of 8-10 giving the body a running start on staying alkaline. The benefits of this water are it's antioxidant properties which nullify free radical scavengers, plus the extra oxygen in the water is supposed to give energy and boost alertness and memory.

Candida detox Next on the plate is to tackle the candida with capyrilic acid and olive leaf extract (for about 2 months). I'm just going to take the recommended dosages on the bottles adding in one teaspoon of l-glutamine powder per day. The glutamine assists in strengthening the gut wall and helps patch any holes left by the yeast (which could lead to leaky gut syndrome and the problems it leads to, such as food allergies and parasites)

Respiration "The biggest acid you make everyday is carbonic acid. Every cell in your body is producing carbon dioxide from the bottom of your feet to the top of your head that is also surrounded by water and the carbon dioxide plus water makes carbonic acid, that's a very simple thing to keep in mind. By looking for acid based balance the amount of acid produced per day must be equal to the amount excreted or nutralized per day. Not happening in your case. The thing that is happening is, if your body can't neutralize or eliminate the acid you stay at that level, you can't go beyond that level of health. If we can get you to learn how to eliminate more acid and neutralize more acid then it frees you up to get to the next level of health.

Blow out the acid, expand the upper rib cage and take long deep inhalations to breathe in more oxyen to neutralize the acids."

Rick demonstrated one of the finest ways of getting more oxygen into the body is to expand the rib cage practicing with a harmonica (which he pulled out and played). "You are respirating—you are doing lung pushups. You are taking the chest cavity, ribs and the lungs and trying to get them to be fantastic. Anyone who plays harmonica is going to have really good lung functioning. Breathing is so much more important then what you are eating. Do 10 deep breaths a day 3x's a day (with long exhalation), in one month you are going to have a respiratory function that is 10%-15% better then you have now. Which means 10%-15% more energy and 10-15% less fermentation because fermentation only takes place where there is no oxygen. CO2 is the biggest acid, focus on the exhale to get that acid out. It's the greatest tool you have and it's free."

Alkalizing with baking soda "The methods of getting rid of acidity from the body are respiration, perspiration, defecation and urination. Those are the four channels. If you can't eliminate the acids it's a really good idea to neutralize the acids. Take a 1/16 of a tsp. bicarbonate of soda every hour, and take it under the tongue so it bypasses the digestive system."

These past few months I've been slacking on checking and maintaining my body's alkalinity, so I'm back on it. Rick gave me a great little tool (from the vet) that allows me to carry the soda in my back pocket making it more convienient to take. The little 1/16 tsp. of baking soda will help the system remain in range more than the one tsp. a day method. According to my calculations, there are approx twenty-two 1/16 servings to a tsp. of baking soda, so one can easily be popping it throughout the day.

Bentonite clay taken internally in the morning upon rising to detox. This is problematic for those of us taking supplements upon rising which are not supposed to be taken with clay. However, I'm trying to fit it in somewhere along the line.

Magnesium oxide also called Oxy-Mag (NOW makes it). Helps with alkalinity and to oxygenate the system. I'm using it as a detox to clean/improve bowel function. The process of oxygenating the bowel breaks down and eliminates solidified waste lodged in the large and small intestines. This is much like a colonic without the cost. Beware, if you take it (up to one teaspoon at night in water with the juice of 1-2 lemons), you must leave time the next morning to eliminate fully. In this way, this process is much like having an enema. Stool will be watery, even explosive. Rick says that eventually this watery stool will cease when the body is cleaned of accumulated waste. Make sure to repopulate with probiotics. I try and do this once a week.

Food During our session together I became increasingly confused about food. Rick pointed out that although my diet was pretty good, it was still heavily carbs. All those veggies will eventually turn to sugar so they have to be balanced out with oils and proteins. I was told by Nancy that each meal should have a carb, oil and protein and have been following that for some time. Rick has said the same thing and suggested I start the day with a chia smoothie.

"The perfect breakfast would be 2 tablespoons of chia seeds, ground to be chia meal with 2 tablespoons of chlorophyl, (spirulina, chlorella or grass powders (greener grasses, alkavision greens). Ground flax seeds can be added."

"Chlorophyll is very cleansing and is a blood builder and it has a molecular structure that looks almost identical to red blood cells. It instantly gives nourishment to the blood. When you take the chia seed and the flax seeds which are 28% protein 40-50% omega 3 fatty acids, that's very little carbohydrate there. The greens are 100% carbohydrate and when you mix them together, now you have carbs that burn like paper and you've got fats and oils, with proteins that don't really burn. The fats and oils are like the charcoal—now you have a sustained burn going on and then you don't ferment the sugars because the coal, the fatty acids, are there. So that breakfast with the chia seeds or flax seeds, or both, and any of those chlorophyls you are using can be a very beneficial superfood breakfast. So easy and so fast to make. Use a lot of water (12 ounces of water), let this all sit for 10- 20 mins. and keep shaking it up to make sure the water is absorbed. Add the chlorophyll products and it will thicken up. They time release and give it back to you so you have have nice long sustained energy with oils, proteins and carbs all day. You'd be surprised, you don't need a lot of food, what you need is the right food."

Rick suggested the more chia seeds used, the less hungry one will be. He mixes 1/4 cup chia seed in with a quart of vegetable juice and that is his favorite breakfast.

From: http://www.chiaseedsdirect.com/cs5.html
Chia seed benefits include:

• High In Omega-3 Acids. Chia seed has higher levels of alpha-linolenic acid (ALA) than flax seed. ALA is an essential acid because it is not produced by the body. In fact, chia seeds have the highest known whole-food source levels of Omega-3 acids, as measured by percent of weight.
Rich In Antioxidants. Chia is a great natural source of antioxidants, including chlorogenic acid, caffeic acid, myricetin, quercetin and flavonols.
• Full of Important Nutrients. Chia is an excellent source of calcium, phosphorus, magnesium, potassium, iron, zinc and copper. Chia contains six times more iron than spinach.
Low In Sodium and Cholesterol-Free. Chia contains less than half the sodium of flax seed, per serving. This is important to those with high blood pressure and concerned about sodium intake. As a plant-based source of Omega-3, chia seed is cholesterol-free.
Promotes Hydration. Chia soaks up water and this promotes hydration and electrolytes retention.
Helps in Weight Loss. Chia is very filling. As more Chia is eaten, there's less room for fattening foods.
Good Source of Fiber. Chia is about 40 percent fiber. A daily serving of chia seeds contains about 11 grams of fiber. About five percent of this fiber is soluble, which can help reduce cholesterol.
• Builds Endurance. The Mayan word for Chia is strength. Chia builds stamina and endurance because it steadily releases slow-burning glucose into the bloodstream.
Gluten-Free. The protein in chia seed is gluten free. Chia seeds are also nonallergenic.
Provides All Essential Amino Acids. Chia provides all eight essential amino acids. Since essential amino acids are not produced by the body, it's important to get amino acids from nutrient-rich sources like chia seeds. The amino acid leucine is abundant in chia seeds. Research suggests leucine may help regulate blood sugar levels and aid in muscle recovery after exercise.
Stays Fresh. Unlike flax seed, chia seed stays fresh for extended periods of time. Chia seeds stay fresh for
24-36 months when stored in proper conditions (cool and dry) .

Nov. 16, 2012 • 2nd Live blood microscopy

I saw Rick for a second live blood analysis on November 16, 2012. There has been significant improvement to the blood. No ghost cells, no rouleax or sticky blood, the red blood cells are fuller, look stronger and are hydrated. The dehydration problem from last session is gone. I'm making a concerted effort to hydrate. There is nice space around the red blood cells and they were plentiful. Candida has been greatly reduced in just 2+ months.


The treatment of olive leaf extract, caprylic acid and l-glutamine powder as per his suggestion for candida is working. I began addressing it by taking one capsule of both the olive leaf and caprylic acid plus one heaping tsp. of l-glutamine in the morning with my other supplements. After I was sure I had no reactions to this, upped the dosage to 2 caps of each with one tsp. of l-glutamine. Eventually, I added in another dose of these three supplements with lunch. So now I take 4 caps of each a day with 2 teaspoons of glutamine powder. Rick recommended I do this for another month then switch to Candex, but my nutritionist tells me that Candex now has corn (the evil demon) in it.
  

My blood no longer looks like it's dying. That is not to say that the Morgellons has been effected, we saw two fibers in the dried blood samples. There seemed to me to be more bacteria in the blood and the white blood cells although they are strong and were very active were not plentiful. I attribute the lower level of white blood cell activity to the fact that I was more sedentary these past two months then normally due to a foot problem that kept me off it. Rick suggested that I do lymphatic drainage work using a brush or mitt, followed by getting on a rebounder to jumpstart the lymph moving. That was a great idea. He feels that getting the lymphatics going will produce more white blood cells to remove the bacteria from the blood.

The chia seeds in the morning are wonderful. I find they curb the appetite, and help with blood sugar regulation. I still need to take food in the morning or my energy levels get wonky and I get manic, but that is probably just me.


Above: Arrows pointing to bacteria. More bacteria being shown below.


Above: Neutrophils  (white blood cells). They were healthy and active but not enough of them. Rick suggests way to activate more white blood cell activity by way of the lymphatic system.

"Dry skin brushing with a natural bristle brush will help loosen things up. More white bloods cells are like having more garbage trucks on the street to pick up the debris. How do we get the lymphatic system to become more stimulated? Rebounding (trampoline) is great and hydration too is important. The cardiovascular system—your blood, is very thin like water and the lymphatic system is a little thicker, more like ketchup or honey and even a slight drop in hydration is going to make that more sluggish. Doing some of the things good for the lymphatics like yoga, stretching—even breathing and stretching in the morning, is actually very helpful. Rebounding is fantastic because you're at 2Gs when you hit bottom bounce and you're weightless when you hit the top.

Basically the lymphatic system drains to the intestinal system to get the fat out of your body. They say (when doing dry brushing) to draw everything to your heart center.

Most people start the dry brushing from their hands and work their way up to the shoulder. Rick described the top of the arm (armpit area) as the mouth of the river and feels dry brushing should begin around the shoulder area to open things up so that by the time you get up to your fingers, the immediate area around the armpit is open for better drainage.

I start here from the top part of the arm down and around the whole armpit area—and those bristles are like shaking the ketchup bottle. It doesn't have to be hard, even a ticklish feeling works. If you start by the shoulder you create a vacuum so by the time you get to your elbow you feel tingling—that's because things are moving. It's moving because you've opened up the mouth of the river.

Same thing with the legs. Move from the big fat part first before going to the extremities (feet), always moving it towards the heart center. Remember, there are a lot of lymph nodes in the neck, in the breast plate and the armpits, so you can do your face in little circles, you can do your breast with a little softer brush if you like. I like the yerba prima because it's nice and stiff but for the face and breast tissue you might want to go to Bed, Bath and Beyond and get a softer natural brush as some prefer a softer approach. I do my hair/head first and I do a lot on my neck and thyroid. Do the dry brush before showering. Whatever brush you use wakes up the body and allows more of the white blood cells to move.

If you understand your vascular system, it has a pump known as a heart and a lot of smooth muscle down the arterial walls that keep everything flowing with pressure through your cardiovascular system. The lymphatic system is more like the drain of your sink. It doesn't have pressure like the cold and hot water and it's passive. Things move by a series of one-way valves and white blood cells once filled with garbage can't go back. They are carrying very toxic stuff, so stretching works, massage works, and dry skin brushing which stimulates everything by way of electrical impulses works. Just by doing these things you find your immune response starts to increase dramatically. It doesn't take much, only about 5 to 7 minutes of dry skin brushing and it wakes you up.

Once the white blood cells fill up (when the garbage truck is full) they go into the lymphatic ducts to be removed from the circulatory system because they are toxic and they are deadly to you once they are full. Then they go to the sewage system to remove it and take it to the sewage treatment plant for processing, your colon, to then be removed from the body completely, so it's isolated from the cardiovascular system. There are lymphatic ducts all over the place and all those little spots where there are ducts and lymph node, those nodes are like little compost piles. The body has millions of them because if they can compost on the spot where there are things that are really bad, it's not so dangerous to move it to the next spot. When the lymphatic system get clogged typically there are things you can do herbally like lymph teas. I like better hydration, because better hydration always helps a great deal with the lymphatics. Number two would be the rebounder, and three would be the dry brushing. The combination of dry brushing before getting on the rebounder is a great one-two whammy (loosen up the ketchup, then get it to move through the system).

This will maybe help a great deal in cleaning up the stuff in the blood because it's adding an unstoppable load to you. Just think of a grocery store when there are always customers going through checkout—there is no end to it. The lymphatic system is the arsenal of housekeepers.

I asked at this point if there were a way to stop the bacteria and Rick replied that being diligent with pH related as well and I think the body is always going to try and keep up the principles of what you are trying to accomplish. (Pick up at 11:13)"


Above: Candida – Arrow on top is candida being pushed off the red blood cell. Arrow below is candida free-floating in the blood. There has been a major decrease in the amount of candida in the blood after 2 months of treatment with olive leaf extract, caprylic acid and l-glutamine but still have more to go.


Above: cluster of candida (yeast).


Above: Pteroharps Bacterial cells inappropriately called blood clotting cells, or platelets with crystallized urine around them, bacterial cells that live in their own waste product.



Above: The elephant in the room. Fiber floating in dried blood sample.


Above: 2nd fiber. Red blood cells cling to it.


Above: 2nd fiber enlarged.


Above: 2nd fiber in super-duper enlargement.


Dried blood. Looks like a fiber clinging to it.


Above: Liver is okay.


Above: To come. More information will be added as I finish transcribing the tapes.

Rick supplied the following charts for those that want to download them. To the right, Visual Reference Guide to Live Blood Analysis.

Download the chart below by clicking on it.

Download the chart below by clicking on it.

Below images were submitted by ColoradoGirl so that we may start to see what patterns might be showing up among Morgellons sufferers. The images contents were the notes I was given and were not from Rick. Thanks CG for the use of these! Anyone else wishing to submit their live blood microscopy for this page, please email me.


Above: The arrows are pointing to yeast. Ghost cells in the background. Black particles are undigested food.


Above: Fungal/mold colony. The white spheres above it are yeast (candida).


Above: Candida and black mold combining.


Above: White blood cell engulfing mold.


Above: Necrosis Dying red blood cells look like donuts. Blood is acidic.


Above: Arrow on the left is pointing to white blood cell engulfing mold and candida. Right arrow, candida and mold.


Above: White blood cells trying to engulf colonies of mold and candida. Bottom left clump looks like Heterogeneous symplasts.


 Above: Arrow to the far left, white blood cell. Arrow towards the middle, yeast (candida), arrow to the right, mold. Mold and candida tend to clump. Lots of ghost cells in the background.


Above: Mold and more candida/mold clump as well as white blood cells trying to do their job.


Above: Parasites in the red blood cells.


Above: .


Above: Fibrous Thallus or Colloid Symplast (this is the large floating particle in the image) indicates a nationalization of "garbagelike" bacteria, yeast/fungus, mold and their acid wastes and acid crystals lying in a dormant/inactive state. The diet is too high in protein, carbohydrate (sugar), junk food, experimentation with recreational drugs and or use of prescription drugs. Disregard the pointing arrow for now. Need to get clarification on it.


Above: Mold, candida, parasites. The arrow is pointing to a rod shape that was wiggling .

Below: An anonymous submission of a woman with Morgellons.

Note the similarity to the fibers in my blood.














Below: Images that come from a woman in the U.K. taken after three years of undiagnosed skin and scalp problems of itching, and crawling sensations. In comparison with the above live blood images, we again see more of the same problems in the components of the blood and the presence of bacteria, yeasts and fibrin. 


Above: In the center, a white blood cell that has captured a great many bacteria it is not very big and not well formed. Note two single bacteria showing as black dots in the upper middle edge of image. Red blood cells surrounding the white blood cell small and weak, malformed, and stuck together (Rouleaux) and not well oxygenated.


Above:  Red blood cells demonstrating Rouleaux—spread like scattered stacks of coins and stuck together. Some are small and weak and show bacteria infiltrating the lipid layers (black dots at or just inside the edges), many have irregular edges giving a lumpy appearance where bacteria has breached the membrane. One in particular, on the right side of image middle, is a small old red blood cell that has been very damaged by bacteria and lumpy in appearance. A second very damaged red blood cell is just to the right on the edge of the image.
Bacteria can be seen throughout the sample as black dots.


Above:  Red blood cells after having no fats or oils in diet for one month while supplementating with Vit C, multiple B's and Iron: healthy size nicely round and plump, no Rouleaux, no fibrin. Note the lipid layers as seen by white halo around each cell—thick and intact.


Above:
Red blood cells very round and healthy with intact and strong lipid layers.
In center, one aggressive bacteria moving in on a red blood cell.


Above:
White blood cell not very healthy, center, with captured bacteria.
Above on left, large red blood cell with intact lipid layers, and on right smaller
and weaker red blood cell with thinner lipid layers.


Above: Red blood cells. Top left, very healthy and strong. Others weaker, smaller.
Fibrin strands left bottom edge. Bacteria lower right, seen as a black dot.

To watch the videos of Kate's live blood analysis follow the links below. Once you get onto her video page, you can negotiate by clicking on the 'next' button.


Watch white blood cells swim with microscopic ‘paddles’

Cells have places to be, and they have all evolved different ways to get there: Red blood cells change their shape, and bacteria use whiplike appendages to propel themselves forward. Now, new research suggests white blood cells have their own special way of swimming, which biologists have dubbed “molecular paddling.”

For years, scientists thought white bloods cells could move across 2D surfaces, like blood vessels or skin layers, only by attaching to and crawling along them. They also knew certain human and mouse white blood cells could swim in liquids, but they weren’t sure how.

To find out, researchers recorded how human white blood cells, or leukocytes, moved in liquids and on solid surfaces. Videos suggested the cells swam by changing their shape and using a breaststrokelike motion. But such movement wasn’t enough to explain the speeds clocked by the cells. So the scientists came up with a new proposal: The cells’ membranes are packed with protein “paddles” that give them a speed boost.

As the leukocytes swam, researchers saw protruding proteins migrate from the front to the back the cell, as if on a treadmill (above). According to their hypothesis, as the cell membrane moves backward, these proteins contract and propel the cell forward. Then, when the proteins reach the back of the cell, they migrate inside the membrane and move back out to the front to start the process over again.

And because the proposed paddling proteins moved internally from the rear to the front of the cell as it swam, the researchers suspect the proteins are “recycled,” allowing the cell to continuously paddle, they reported online last month in Biophysical Journal . Don’t expect a speedy journey, though. This novel paddling mechanism is about four times slower than crawling.


Talk Overview

In this talk about mobile microscopy, Daniel Fletcher describes work from his lab to make small and inexpensive microscopes that can be project images to cameras on cellular phones. These mobile microscopes can be used for medical diagnostics in the field.

Questions

  1. Mobile microscopes in this talk refer primarily to:
    1. Microscopes that are used in teaching vans and brought to schools
    2. Smaller versions of confocal microscopes
    3. Compact magnifying optics that can be coupled to cell phone cameras
    4. Confocal microscopes that can be disassembled and packed in suitcases

    Answers


    Biology Labs Two and Three: Microscopy

    Ocular reticles are calibrated by using a tiny 2.0 mm ruler called a stage micrometer
    - Once the absolute distance of the stage micrometer that corresponds to the distance between adjacent lines of the reticle is determined for each objective lens power, that relationship can be used as a converting factor for each objective lens magnification to determine the absolute length

    The closest distance that 2 distinct parallel edges in the object can be separated and still be perceived as separate edges in the image produced by the lens ability of a microscope to produce an image in which 2 objects separated by a short distance can be seen as 2 objects rather than one

    The lower the resolution limit number, the better the resolution (the shorter distance between two objects that can be identified as 2 separate points rather than 1)
    - Shorter the resolution distance, the finer the details that can be discerned from the image

    Resolution distance in a light microscope is impacted by 2 factors: NA (bigger is better) and wavelength of light (shorter is better)


    WATCH: Your mouth is full of bacteria blooming. And it’s beautiful

    The thin plane of laser light is useful in illuminating living cells because concentrated light is fast and gentle enough to capture action without cooking cell innards, while also helping to preserve the fluorescent dyes used to label structures within the cell.

    That lattice-light sheet alone can’t see very far beyond the very surface of a cell, however. “If you look at any depth inside of anything like the zebrafish or something like that, it’s too turbid to see,” said Betzig. “It’s just a mess.”

    To deal with that inner mess, the researchers used a technique inspired by the distant stars. Astronomers use something called “adaptive optics” to cancel out the visual distortions of our turbulent atmosphere. They harness a “guide star” (a bright, nearby star or an artificial laser shot skyward) as a predictable reference point.

    Scan laser guide stars into a specimen, and you have a way to track and de-mess the light’s bends and twists as it moves through living tissues.

    A view of the adaptive optics corrections applied to the skeletal rod supporting a growing zebrafish. T. Liu et al./Science 2018

    Betzig described first seeing cells with only the lattice sheet technology in 2014 as “watching a lion in a zoo.” With the new, combined scope, he’s now “watching the lion on the savanna.”

    Galbraith takes the metaphor even further: “You’re not just watching it on the savanna, you’re lying in wait on the savanna to see what actually happens.” She said that for microscopists, the fundamental challenge has always been to observe and not disturb, and that the new microscope goes a long way toward doing that.

    The next step is making more than one working version of it.

    “When you see these movies, you just say, ‘Oh my goodness, I want to study this,’” said Jennifer Lippincott-Schwartz, a senior group leader at Janelia and a colleague of Betzig’s. “But a key aspect of whether it will be embraced, is how quickly people can have access to these microscopes.” She added: “They’re not easy to build.”

    Betzig is working to commercialize the technology in the coming years, but he fears that even if there was a version a scientific lab or a pharmaceutical company could buy, there would still be a big data problem. The microscope doesn’t just spit out pretty video ready for posting to your favorite science social media account. What you get out of the machine is an unholy pile of flat images that need to be laboriously re-stacked through space and time.

    Once you get through the pain and anguish of doing that, the slow reveal, said Betzig, blows away the researchers he’s working with, without fail. But many are left “crying a month later when they don’t know how to deal with 10 terabytes of data.”

    He’s trying to solve that data problem with his feet. This summer Betzig will move his lab to the University of California, Berkeley, in part to try to tap local tech expertise.

    “I’m going everywhere through the Bay Area banging the drum, trying to marshal troops together to work on it because, face it, and I’m just too goddamned old to solve the problem myself.”

    If he doesn’t succeed? Betzig is toying with the idea of harnessing his microscope to create salable art in his retirement. Think George W. Bush, the oil painter, just with lasers and tissue samples.


    Science in Focus: How Do White Blood Cells Move So Fast?

    If you fall and scrape a knee, it’s the job of white blood cells called neutrophils to rush to the site of infection and chase down invading bacteria.

    Science in Focus

    This story is part of a series highlighting the beauty of science at UCSF and the groundbreaking research taking place behind each image.

    In order to race after bacteria at speeds up to a thousand times that of most human cells, neutrophils must move in a special way.

    Lillian Fritz-Laylin, PhD, a postdoctoral scholar in UC San Francisco’s Department of Cellular and Molecular Pharmacology, and Megan Riel-Mehan, PhD, a postdoctoral scholar in the Department of Bioengineering, want to know how that works.

    “Our immune system relies on fast cells like neutrophils getting to where they need to be,” said Fritz-Laylin. “But we know surprisingly little about how these fast cells move.”

    Fritz-Laylin is a cell biologist. Riel-Mehan is a visualization scientist who develops tools to process and display complex data.

    Fritz-Laylin used a fast, high-resolution lattice light sheet microscope to record 3-D images of neutrophils migrating through a fibrous matrix. But the recorded data were enormous and complex. “I couldn’t open even a single frame of one movie on my laptop without a software crash,” Fritz-Laylin said.

    Lillian Fritz-Laylin, PhD

    That’s where Riel-Mehan came in. She processed and rendered the images, drawing on her animation background and developing new software tools to shade crevasses and allow a human viewer to visually understand the 3-D data.

    The rendered videos revealed that neutrophils create two types of protruding “feet” to help them move: planar sheets and multi-petaled rosettes. Previously, scientists thought planar sheets could only form when cells were grown in flat dishes, but the new videos show that planar sheets form even in a 3-D matrix that resembles the cells’ natural environment.

    This was the first time that researchers were able to watch up close as neutrophils moved in three dimensions.

    Megan Riel-Mehan, PhD

    Now, Fritz-Laylin is investigating how these protrusions are built and how they mechanically propel neutrophils forward, allowing them to race through our bodies and chase down invading microbes.

    Fritz-Laylin and Riel-Mehan worked with a team of biologists, microscopy specialists, and data scientists to produce this video: Dyche Mullins, PhD, and Graham Johnson, PhD, of UCSF Bi-Chang Chen and Eric Betzig of Janelia Research Campus and Tom Goddard of UCSF Chimera.

    “This project exemplifies the wonderful interplay that can exist between experimental science and visualization science,” Riel-Mehan said. “It makes the case that visualization should be part of the research process, not just something you do at the very end for an article cover.”


    What microscope is used to study the internal ultrastructure of cells?

    Regarding this, what type of microscope would a biologist use to study the ultrastructure of cells?

    Two types of electron microscopy&mdashtransmission and scanning&mdashare widely used to study cells. In principle, transmission electron microscopy is similar to the observation of stained cells with the bright-field light microscope.

    Furthermore, what type of microscope would be most effective for studying a living cell? Compound microscopes are light illuminated. The image seen with this type of microscope is two dimensional. This microscope is the most commonly used. You can view individual cells, even living ones.

    what kind of microscope should be used to study the internal structure of a white blood cell?

    Transmission electron microscopes are designed to examine the internal structures of a cell, whereas a scanning electron microscope only allows visualization of the surface of a structure.

    What methods would you use to study the structure of eukaryotic cells?

    Microscopes, spectroscopes, centrifuges to separate cellular components, all used to study the structure of eukaryotes. Cell Biologists who are not microbiologists are completely concerned with eukaryotic function, structure, etc. So eukaryotic structure can be studied in a cell biology laboratory.


    Incredible 3D Video Microscopy Shows Human White Blood Cells Use Molecular Paddles to Swim

    Human white blood cells, known as leukocytes, swim using a newly described mechanism called molecular paddling, researchers report in the Biophysical Journal. This microswimming mechanism could explain how both immune cells and cancer cells migrate in various fluid-filled niches in the body, for good or for harm.

    “The capacity of living cells to move autonomously is fascinating and crucial for many biological functions, but mechanisms of cell migration remain partially understood,” says co-senior study author Olivier Theodoly of Aix-Marseille University in France. “Our findings shed new light on the migration mechanisms of amoeboid cells, which is a crucial topic in immunology and cancer research.”

    Cells have evolved different strategies to migrate and explore their environment. For example, sperm cells, microalgae, and bacteria can swim through shape deformations or by using a whip-like appendage called a flagellum. By contrast, somatic mammalian cells are known to migrate by attaching to surfaces and crawling. It is widely accepted that leukocytes cannot migrate on 2D surfaces without adhering to them.

    A prior study reported that certain human white blood cells called neutrophils could swim, but no mechanism was demonstrated. Another study showed that mouse leukocytes could be artificially provoked to swim. It is widely thought that cell swimming without a flagellum requires changes in cell shape, but the precise mechanisms underlying leukocyte migration have been debated.


    This is a 3D videomicroscopy of the cytoskeleton of a swimming lymphocyte showing protrusions traveling along cell body that mimic a breast-stroke motion. Credit: SoSPIM microscopy: L. Aoun, O. Theodoly, M. Biarnes, R. Galland

    In contrast to previous studies, Theodoly, co-senior study author Chaouqi Misbah of Grenoble Alpes University, and their collaborators provide experimental and computational evidence in the new study that human leukocytes can migrate on 2D surfaces without sticking to them and can swim using a mechanism that does not rely on changes in cell shape. “Looking at cell motion gives the illusion that cells deform their body like a swimmer,” Misbah says. “Although leukocytes display highly dynamic shapes and seem to swim with a breast-stroke mode, our quantitative analysis suggests that these movements are inefficient to propel cells.”

    Instead, the cells paddle using transmembrane proteins, which span the cell membrane and protrude outside the cell. The researchers show that membrane treadmilling — rearward movement of the cell surface — propels leukocyte migration in solid or liquid environments, with and without adhesion.

    However, the cell membrane does not move like a homogenous treadmill. Some transmembrane proteins are linked to actin microfilaments, which form part of the cytoskeleton and contract to allow cells to move. The actin cytoskeleton is widely accepted as the molecular engine propelling cell crawling. The new findings demonstrate that actin-bound transmembrane proteins paddle and propel the cell forward, whereas freely diffusing transmembrane proteins hinder swimming.


    This video shows imaging of the backward treadmilling of paddling molecules outside the cell. Credit: TIRF microscopy: N Garcia-Seyda

    The researchers propose that continuous paddling is enabled by a combination of actin-driven external treadmilling and inner recycling of actin-bound transmembrane proteins through vesicular transport. Specifically, the paddling proteins at the rear of the cell are enclosed inside a vesicle that pinches off from the cell membrane and transported to the front of the cell. By contrast, the non-paddling transmembrane proteins are sorted out and do not undergo this process of internal recycling through vesicular transport.

    “This recycling of the cell membrane is studied intensively by the community working on intracellular vesicular traffic, but its role in motility was hardly considered,” Theodoly says. “These functions of protein sorting and trafficking seemed highly sophisticated for swimming. Our investigations, to our own surprise, bridge such distant domains as the physics of microswimmers and the biology of vesicular traffic.”

    The authors say that molecular paddling could allow immune cells to thoroughly explore all locations in the body as they migrate in liquid-filled niches such as swollen body parts, infected bladders, cerebrospinal fluid, or amniotic fluid. Moving forward, the researchers plan to investigate the functions of molecular paddling in various environments and assess whether other types of cells use this mode of migration.

    Reference: “Amoeboid Swimming Is Propelled by Molecular Paddling in Lymphocytes” by Laurene Aoun, Alexander Farutin, Nicolas Garcia-Seyda, Paulin Nègre, Mohd Suhail Rizvi, Sham Tlili, Solene Song, Xuan Luo, Martine Biarnes-Pelicot, Rémi Galland, Jean-Baptiste Sibarita, Alphée Michelot, Claire Hivroz, Salima Rafai, Marie-Pierre Valignat, Chaouqi Misbah and Olivier Theodoly, 11 August 2020, Biophysical Journal.
    DOI: 10.1016/j.bpj.2020.07.033

    The work was supported by the French Agence Nationale de la Recherche, the LABEX INFORM, the Région Sud, the Turing Centre for Living systems, the Excellence Initiative of Aix-Marseille University-A*MIDEX, a French ”Investissements d’Avenir” program, and the company Alveole.


    Wallace Marshall

    Wallace Marshall received his BE in Electrical Engineering and his BS in Biochemistry from SUNY Stony Brook and his PhD in Biochemistry from the University of California, San Francisco. He was a post-doctoral fellow at Yale University. Currently, Marshall is a Professor of Biochemistry and Biophysics at UCSF. His lab is interested in the… Continue Reading


    Watch the video: εικονες μικροσκοπίου 2 (June 2022).


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